PrimerExplorer is a primer designing software, which is specifically for LAMP, a novel gene amplification method. The features are listed below. 1) Can easily design 4 primers based on 6 regions required by the LAMP method. 2) Can also design loop primers. 3) Use the "nearest neighbor" method for Tm calculation. 4) Installation is unnecessary since it can be used via the web. About LAMP method "LAMP" which stands for Loop-mediated Isothermal Amplification is a simple, rapid, specific and cost-effective nucleic acid amplification method solely developed by Eiken Chemical Co., Ltd. It is characterized by the use of 4 different primers specifically designed to recognize 6 distinct regions on the target gene and the reaction process proceeds at a constant temperature using strand displacement reaction. Amplification and detection of gene can be completed in a single step, by incubating the mixture of samples, primers, DNA polymerase with strand displacement activity and substrates at a constant temperature (about 65°C). It provides high amplification efficiency, with DNA being amplified 109-1010 times in 15-60 minutes. Because of its high specificity, the presence of amplified product can indicate the presence of target gene. For further information on the LAMP method, please visit Eiken GENOME SITE. PrimerExplorer operating environment and cautions OS PrimerExplorerV4:Windows Vista/7 Browser PrimerExplorerV4:Internet Explorer 7/8/9 Recommended Environments Since the calculation will be done on users' computers, the PC specifications above are recommended. If the PC requirements are not met, it might cause serious lags and bad performance. Fire Wall or similar software might interfere and block the Java program from correctly transferring data.