PrimerExplorerV5 Software
  1. Click on [Browse] button.
    Choose and upload the target sequence file.
    Following formats can be used.
    • Plain text format (sequence only)
    • FASTA format
    • GenBank format
    • Multiple alignment file format
    • Target sequence save file format

  2. Choose the corresponding parameter set.
     
  3. Click on the [Primer Design] button
  1. Click on the [Browse] button.
    Choose the Primer Information File.
     
  2. Click on the [Primer Design] button.
 
Target Sequence File/Primer Information File

 
Parameter Set(not applicable to the loop primer design.)

 Automatic Judgment
 Normal
 User Assignment

         

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