PrimerExplorerV4 Software
Operation under the primer design
1. Click on [Browse] button.
Choose and upload the target sequence file.
Following formats can be used.
  • Plain text format (sequence only)
  • FASTA format
  • GenBank format
  • Multiple alignment file format
  • Target sequence save file format
2. Choose the corresponding parameter set.
3. Click on the [Primer Design] button
Operation under the loop primer design
1. Click on the [Browse] button.
Choose the Primer Information File.
2. Click on the [Primer Design] button.
Target Sequence File/Primer Information File
Parameter Set(not applicable to the loop primer design.)
Automatic Judgment
User Assignment

Cautions for use
Java Runtime Environment(JRE) 1.6.0_33 is needed for using PrimerExplorer.
To page top