[1]Introduction (operating environment) [2]Operation of each screen (designing regular primers/designing loop primers) [3]Appendix [4]A Guide to LAMP primer designing (Manual) No.1(2.1MB) No.2(2.0MB) No.3(1.7MB) PrimerExplorer operating environment and caution Before using this software, activate the Java™ and Java Script function of the web browser. This software is specifically for designing the primer sets for Loop-mediated Isothermal Amplification (LAMP) method. This software generates the primer sets based on the target sequence information, which meets the primer designing requirements. One primer set contains 4 primers, FIP (Forward Inner Primer), F3, BIP (Backward Inner Primer) and B3. FIP (BIP) consists of the sequence of the F1c (B1c) and F2 (B2) regions. F1, F2, F3 are about 20bp long sequences selected from the target gene, B1, B2, B3 are about 20bp long sequences selected from the complementary strand. F1c and F1, B1 and B1c are complementary regions. The position of primers on the target gene is as follows: This software can also design the loop primer, which is located between F1c and F2c, or B1c and B2c. Operating Environment 　　　　　Verified Operating Environment Case1 OS Windows 7 / 8.1 / 10 Browser Internet Explorer 10/11 　　　　　Verified Operating Environment Case2 OS Mac OS X 10.8.2 Browser Safari 6.0.1 　　　　　 Recommended Environments The PC environments mentioned above are recommended. Please utilize to activate the Java™ and Java Script function of the web browser. In the case of valid of a pop-up blocking, please set to permit the pop-up in the primerexplorer.jp site. Network restrictions The FireWall setting might restrict the operation of primerexplorer.jp site. In this case, please consult your LAN administrator. [1]Introduction (operating environment) [2]Operation of each screen (designing regular primers/designing loop primers) [3]Appendix [4]A Guide to LAMP primer designing (Manual)：in preparation